It may be worth (if you're up to it) taking the actuator head off the motorised valves and manually turning the spindle to the open position on the valve itself and repeating your test.
This way, the motorised head will remain in the shut position and you can be sure whether the fact that the boiler will not fire is due to lack of flow, or whether it is down to the fact that, by opening the valve using the 'latch', you are actuating a valve microswitch.
I've known a bypass valve fail to shut properly, but to jam shut seems unlikely. That said, at 2.5 (metres head?) this may exceed the head your pump is running at by a long shot on a modern system. Looking at performance curves for the Alpha 2L range as an example of a reasonably modern pump, I can see that 2.5m head is only achieved in a constant pressure setting, or on the higher fixed-speed settings. On proportional pressure settings, you'll not get 2.5m head in no-flow conditions, and, in any case, a differential pressure automatic bypass valve will not work correctly with proportional pressure settings. Surprising how many installers cannot get their heads around this.