Apologies, yes it’s open and on the return coil pipe
Turn boiler up a bit. It won't alter this so you'll notice but it will be better for the boiler. 65 C.
It sounded at first like there was a cross flow (cold to hot). That will have a rapid chilling effect on a cylinder but you seem to have ruled that out.
The stat works and from what I can tell, looks to be reading close enough and the valve can be heard to close when you turn the stat down, which tells me it opened.
The gate valve is open so there should be enough flow through the coil to heat the cylinder in one hour.
If the boiler is running at 60-65, then it should heat up OK.
If the flow from the boiler is being shared with something else, such as under floor heating, radiator circuits etc. Then they can in some cases rob the coil of heat. They would have to be open though and obviously will be warm/hot. You say they are off when trying to heat the cylinder? I suppose we can rule that out too?
If the flow rate through the coil is restricted in some way, you won't get the heat loss from coil to secondary (tap) water in the cylinder. That is unlikely on a new cylinder install. The honeywell valve looks to be the right way round and pipes are 22mm.
No water running through tundish (back right black thing with opening in)?
Thinking out loud as well as clutching at straws. The frustrating thing is that I know it's something that would be diagnosed on site and probably fairly easily too.