I'm thinking that if those rads are not working and there is some sort of restriction in the flow around the system, it is a good place to start. As good a place as any.
You will confirm whether there is a clear path to each end of each rad.
It will only tell you if that length of pipe to the rad is clear of course. Beyond that is more difficult, it can flow from either side of a tee, for example.
You may be better releasing the pressure from the rad in your case, that way there will still be pressure in the system to allow water to be pushed from the end you have disconnected. You will have to keep it topped up too.
Once you've done that and ruled out a blockage in each section, you can move on with your thinking.
A lot depends on route too and like I said earlier, we haven't seen the job.
If for example, the two downstairs rads are fed together then a blockage in either flow or return would stop them both from working but if they are both tee'd off separately, you are less likely to learn anything because water can flow form either direction.
I am just trying to think of a good place to start !
Hope this is helping and not confusing the issue.